Part:BBa_K861024:Experience

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Applications of BBa_K861024

• iGEM2012 WHU-China In the in vitro assay we performed, we collected cell extracts from 1L medium and got protein solution of high concentration. The enzyme assay was then performed to measure the ability of degrading fatty acids by single enzyme and their different combinations.The remaining amount of fatty acids in the medium was measured by the cupric-acetate reaction. In this picture, the color of the sample D standing for cell extracts collected from bacteria overexpressing fadE was lighter than the control(tube A). It means the fatty acids remaining in the tube was less. From the results above, we have proved that the biobrick responsible for overexpressing the mentioned gene can metabolize fatty acids much faster. For more details, please visit our wiki http://2012.igem.org/Team:WHU-China

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